Chandan Hospital Blood Bank
MD (Transfusion Medicine)
Blood bank at Chandan Hospital is a center where blood gathered as a result of blood donation is stored and preserved for later use in blood transfusion.
Whole blood or blood with RBC, is transfused to patients with anaemia/iron deficiency. It also helps to improve the oxygen saturation in blood. It can be stored at 1.0 °C-6.0 °C for 35–45 days.
Platelet transfusion, is transfused to those who suffer from low platelet count. This can be stored at room temperature for 5–7 days. Plasma transfusion is indicated to patients with liver failure, severe infections or serious burns. Fresh frozen plasma can be stored at a very low temperature of -25 °C for up to 12 months.
Collection and processing:
Certain standards are set for the collection and processing of each blood product.
"Whole blood" (WB) is the proper name for one defined product, specifically unseparated venous blood with an approved preservative added.
Most blood for transfusion is collected as whole blood. Autologous donations are sometimes transfused without further modification,
however whole blood is typically separated (via centrifugation) into its components, with red blood cells (RBC) in solution being the most commonly
used product. Units of WB and RBC are both kept refrigerated at 33.8 to 42.8 °F (1.0 to 6.0 °C), with maximum permitted storage periods (shelf lives)
of 35 and 42 days respectively. RBC units can also be frozen when buffered with glycerol, but this is an expensive and time-consuming process, and is rarely done.
Frozen red cells are given an expiration date of up to ten years and are stored at −85 °F (−65 °C).
The less-dense blood plasma is made into a variety of frozen components,
and is labeled differently based on when it was frozen and what the intended use of the product is.
If the plasma is frozen promptly and is intended for transfusion, it is typically labeled as fresh frozen plasma.
If it is intended to be made into other products, it is typically labeled as recovered plasma or plasma for fractionation.
Cryoprecipitate can be made from other plasma components. These components must be stored at 0 °F (−18 °C) or colder,
but are typically stored at −22 °F (−30 °C). The layer between the red cells and the plasma is referred to as the buffy
coat and is sometimes removed to make platelets for transfusion. Platelets are typically pooled before transfusion
and have a shelf life of 5 to 7 days, or 3 days once the facility that collected them has completed their tests.
Platelets are stored at room temperature (72 °F or 22 °C) and must be rocked/agitated. Since they are stored at
room temperature in nutritive solutions, they are at relatively high risk for growing bacteria.
Most collection facilities as well as hospital blood banks also perform testing to determine the blood type of patients and to identify
compatible blood products, along with a battery of tests (e.g. disease) and treatments (e.g. leukocyte filtration) to ensure or enhance quality.
The increasingly recognized problem of inadequate efficacy of transfusion is also raising the profile of RBC viability and quality.
Storage and management:
Routine blood storage is 42 days or 6 weeks for stored packed red blood cells, by far the most commonly transfused blood product, and involves refrigeration but usually not freezing.Transfusions of platelets are comparatively far less numerous, but they present unique storage/management issues. Platelets may only be stored for 7 days, due largely to their greater potential for contamination, which is in turn due largely to a higher storage temperature.
Insufficient transfusion efficacy can result from red blood cell (RBC) blood product units damaged by so-called storage
lesion—a set of biochemical and biomechanical changes which occur during storage.
With red cells, this can decrease viability and ability for tissue oxygenation.
Although some of the biochemical changes are reversible after the blood is transfused, the biomechanical changes are less so, and rejuvenation products
are not yet able to adequately reverse this phenomenon.
Current regulatory measures are in place to minimize RBC storage lesion—including a maximum shelf life (currently 42 days), a maximum auto-hemolysis threshold
and a minimum level of post-transfusion RBC survival in vivo (currently 75% after 24 hours).
Platelet storage is a very different phenomenon from RBC storage, due largely to the different functions of the products and purposes of the respective
transfusions, along with different processing issues and inventory management considerations.
Apheresis is a medical technology in which the blood of a person is passed through an apparatus that separates out one particular
constituent and returns the remainder to the circulation. It is thus an extracorporeal therapy.